Starch Waste as a Substrate for Amylase Production by Sago Effluent Isolates Bacillus subtilis and Aspergillus niger

Sago effluent contains large amount of organic material which has a potential to affect the fertility of soil in agricultural lands when disposed off. In order to reduce this problem and make it beneficial, an experiment was conducted using sago effluent isolates Bacillus subtilis and Aspergillus niger. In present study, an attempt was made to convert the biodegradable sago into useful product by microbial degradation. During the process sago waste with Bacillus subtilis or Aspergillus niger, showed the maximum amylase production of 9.95 mg/ml/min at a pH of 7 and constant temperature of 37°C and maximum amylase production of 9.7 mg/ml/min at a pH of 5 and constant temperature of 20°C. From the result, this study concluded sago effluent as cheap substrate for amylase production, compared to wheat.